Yeast as a Versatile System for Heterologous Protein Expression; Genetically Modified Yeast Strain to Express Human Drug Metabolism Enzymes

Another important factor required for desired expression of a heterologous gene is the choice of correct host system as explained above. Mammalian cells as hosts for heterologous expression is best for the expression of human genes but unfortunately this is associated with low yields, high cost and long generation times. On the other hand prokaryotic system, although results in high protein yields in considerably less time but lacks the posttranslational modifications and processing required for most human proteins.